Home > Explore Data & Reports > Histological Techniques for Marine Bivalve Molluscs: Update


Kim, Y., K.A. Ashton-Alcox, and E.N. Powell. 2006. Histological Techniques for Marine Bivalve Molluscs: Update. NOAA Technical Memorandum NOS NCCOS 26. Silver Spring, MD. 76 pp.

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NOAA Technical Memorandum

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This chapter describes the procedures for determining the reproductive stage of oysters, mytilid mussels, and dreissenid mussels collected for NOAA’s National Status and Trends Mussel Watch Project. Analyses are conducted on paraffin-embedded tissues sectioned at a 5-µm thickness and stained using a pentachrome staining procedure. Each slide is examined microscopically to determine the animal’s sex and stage of gonadal development. A semi-quantitative ranking is assigned.

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